An enzymatic method is described for determination of total serum cholesterol by use of a single aqueous reagent. The method requires no prior treatment of sample and the calibration curve is linear to 600 mg/dl. Cholesterol esters are hydrolyzed to free cholesterol by cholesterol ester hydrolase (EC 3.1. 1.13).

How is serum total cholesterol calculated?

Add your HDL and LDL cholesterol levels, plus 20 percent of your triglycerides, to calculate your serum cholesterol levels. If you have an LDL of 150 mg/dL, HDL of 35 mg/dL, and triglycerides of 180 mg/dL, your serum cholesterol would be 221 mg/dL. That's considered a borderline high level.

What is serum total cholesterol?

A person's serum cholesterol level represents the amount of total cholesterol in their blood. A person's serum cholesterol level comprises the amount of high-density lipoprotein (HDL), low-density lipoprotein (LDL), and triglycerides in the blood. Triglycerides are a type of fat bundled with cholesterol.

Which apparatus are used for determination of serum total cholesterol?

The determination of cholesterol level in human serum by enzymatic colorimetric method was performed using the LAMBDA 465 UV/Vis spectrophotometer and the UV Lab software.

How was the serum sample prepared for the estimation of cholesterol?

Blood serum was prepared by centrifugation at 3000 rpm for 5 min at 4°C and the serum was used as the sample for cholesterol determination. … The activity of cholesterol oxidase and cholesterol esterase was determined by the modified method of Allain et al. (1974).

What is enzymatic determination?

An enzymatic method is described for determination of total serum cholesterol by use of a single aqueous reagent. The method requires no prior treatment of sample and the calibration curve is linear to 600 mg/dl. Cholesterol esters are hydrolyzed to free cholesterol by cholesterol ester hydrolase (EC 3.1. 1.13).

What is serum cholesterol HDL ratio?

To calculate your cholesterol ratio, divide your total cholesterol number by your HDL cholesterol number. So if your total cholesterol is 200 mg/dL (5.2 mmol/L) and your HDL is 50 mg/dL (1.3 mmol/L), your ratio would be 4-to-1. Higher ratios mean a higher risk of heart disease.

What happens if serum cholesterol is low?

Although the risks are rare, very low levels of LDL cholesterol may be associated with an increased risk of: Cancer. Hemorrhagic stroke. Depression.